Two‐photon excited lasing of Coumarin 307 for lysozyme amyloid fibrils detection
نویسندگان
چکیده
منابع مشابه
Nanopore analysis of amyloid fibrils formed by lysozyme aggregation.
The measurement of single particle size distributions of amyloid fibrils is crucial for determining mechanisms of growth and toxicity. Nanopore sensing is an attractive solution for this problem since it gives information on aggregates' shapes with relatively high throughput for a single particle technology. In this paper we study the translocation of lysozyme fibrils through quartz glass nanop...
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Formation of large protein fibrils with a characteristic cross β-sheet architecture is the key indicator for a wide variety of systemic and neurodegenerative amyloid diseases. Recent experiments have strongly implicated oligomeric intermediates, transiently formed during fibril assembly, as critical contributors to cellular toxicity in amyloid diseases. At the same time, amyloid fibril assembly...
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We consider nucleation of amyloid fibrils in the case when the process occurs by the mechanism of direct polymerization of practically fully extended protein segments, i.e., beta-strands, into beta-sheets. Applying the classical nucleation theory, we derive a general expression for the work to form a nanosized amyloid fibril (protofilament) constituted of successively layered beta-sheets. Analy...
متن کاملIdentification of the core structure of lysozyme amyloid fibrils by proteolysis.
Human lysozyme variants form amyloid fibrils in individuals suffering from a familial non-neuropathic systemic amyloidosis. In vitro, wild-type human and hen lysozyme, and the amyloidogenic mutants can be induced to form amyloid fibrils when incubated under appropriate conditions. In this study, fibrils of wild-type human lysozyme formed at low pH have been analyzed by a combination of limited ...
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Given the common knowledge that concentrated saline environments are hostile to life because of biomolecule instability, ionic liquids (ILs) (in the sense of ambient temperature liquids consisting entirely of ions) would seem one of the least likely environments for successful studies of biomolecule behavior. Yet ionic liquids are finding increasing application in the biochemical field. Reports...
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ژورنال
عنوان ژورنال: Journal of Biophotonics
سال: 2019
ISSN: 1864-063X,1864-0648
DOI: 10.1002/jbio.201900052